Long non-coding RNA GNAS-AS1 knockdown inhibits proliferation and epithelial–mesenchymal transition of lung adenocarcinoma cells via the microRNA-433-3p/Rab3A axis

Abstract The goal of this study was to demonstrate the functions and specific mechanism long non-coding RNA (lncRNA) GNAS-AS1 in lung adenocarcinoma. Levels lncRNA GNAS-AS1, microRNA (miR)-433-3p, Rab3A were assessed by quantitative real-time PCR (qRT-PCR). target-binding sites miR-433-3p, predicted confirmed bioinformatics tool (StarBase) a dual-luciferase reporter system. Cell proliferation apoptosis checked using MTT flow cytometry, respectively. Additionally, levels apoptosis-related epithelial–mesenchymal transition (EMT)-associated genes A549 cells analyzed qRT-PCR western blot. We found that upregulated, miR-433-3p low-expressed, overexpressed adenocarcinoma tissues cell lines. LncRNA interacted with negatively regulated levels. direct target miR-433-3p. Downregulation remarkably suppressed proliferation, promoted apoptosis, decreased B-cell lymphoma-2 (Bcl-2) expression, enhanced Bcl-2-Associated X (Bax) level, E-cadherin reduced N-cadherin However, inhibitor reversed all these findings. Similarly, inhibitory effects mimic on Rab3A-plasmid. In conclusion, downregulation EMT through miR-433-3p/Rab3A axis.